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Crystal Growth 101 Literature
Are you looking to get started in crystallization? Check out some of our Crystal Growth 101 guides to help you.
Hampton Research Catalog - Volume 17
Pdf version of the most current Hampton Research catalog, Volume 17. Download
Preliminary Sample Preparation
What to do and what not to do while preparing your sample for crystallization. Download
Crystal Growth Techniques
There are several techniques for setting up crystallization experiments (often termed "trials") including sitting drop vapor diffusion, hanging drop vapor diffusion, sandwich drop, batch, microbatch, under oil, dialysis, and free interface diffusion. Here we offer an overview of these crystallization techniques. Download
Hanging Drop Vapor Diffusion Crystallization
The hanging drop vapor diffusion technique is the most popular method for the crystallization of macromolecules. The principle of vapor diffusion is straightforward. A drop composed of a mixture of sample and reagent is placed in vapor equilibration with a liquid reservoir of reagent. Download
Sitting Drop Vapor Diffusion Crystallization
The sitting drop vapor diffusion technique is another popular method for the crystallization of macromolecules. The principle of vapor diffusion is straightforward. A drop composed of a mixture of sample and reagent is placed in vapor equilibration with a liquid reservoir of reagent. Download
Crystallization Under Oil
Crystallization under oil is a method where a small drop of sample combined with the crystallization reagent of choice is pipetted under a layer of oil. This is also known as MicroBatch Crystallization. Oils can also be used as a barrier between the reservoir and the drop in traditional Hanging or Sitting Drop crystallization experiments. This is known as Vapor Diffusion Rate Control. Download
Dialysis Crystallization
Crystallization by dialysis is an easy variation to the typical vapor diffusion method used to grow crystals. In the dialysis method the sample in question is separated from the ³precipitant² by a semi-permeable membrane which allows small molecules such as ions, additives, buffers, and, salts to pass but prevent biological macromolecules from crossing the membrane. Equilibration kinetics depend upon the molecular weight cut-off of the dialysis membrane, the precipitant, the ratio of the volume, the concentration of the components inside and outside of the dialysis cell, and the geometry of the cell. Download
Viewing Crystallization Experiments
How to observe crystallization experiments under the microscope and what you may see in your drops. Download
Crystallization Scoring Sheet
24 well plate format scoring sheet for screening and optimization. Download
Crystallization Screening Report
An easy to use scoring sheet for a 24 well 4 by 6 crystallization plate. Download
Inorganic or Biological Crystals
Is it protein or is it salt? Download
Crystallization Buffers Table
Table of frequently used crystallization buffers and their useful pH range. Download
Temperature as a Crystallization Variable
Temperature can be a significant variable in biological macromolecule and small molecule crystallization. Temperature often influences nucleation and crystal growth by manipulating the solubility and supersaturation of the sample. Download
Using Halides for Phasing Macromolecular Structures
Preliminary research shows that bromide (as well as iodide) can diffuse into protein crystals when soaked with the appropriate solution and can successfully be used for phasing. Download
Sitting Drop Vapor Diffusion Crystallization for Small Molecules and Peptides
The sitting drop vapor diffusion technique for small molecule and peptide crystallization. The principle of vapor diffusion is straightforward. A drop composed of a mixture of sample and reagent is placed in vapor equilibration with a liquid reservoir of reagent. Download
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