Macromolecular X-ray Crystallography Facility
Office of Biotechnology, (515) 294-9818 or 1-800-643-9504 in Iowa, Fax: (515)294-4629, biotech@iastate.edu
Location: 0202 Molecular Biology Building
Hours: 9 a.m. to 5 p.m. weekdays, appointment suggested
Contacts: 515-294-2846 Facility
515-294-2846 Julie Hoy, Manager
515-294-7103 Richard Honzatko, Professor-in- Charge
Fax: 515-294-4629
E-Mail: hoyj@iastate.edu
Web: http://www.biotech.iastate.edu/facilities/XRAY/xray.html
The X-ray Facility of the Office of Biotechnology provides a Rigaku/MSC rotating anode generator for single crystal x-ray diffraction studies, training in crystallography techniques, and computers with crystallography software. Facility services are provided on a fee basis and include consultation on protein purification, crystallization, and crystal optimization, as well as assistance with crystal screening, data collection, data processing and structure determination and analysis. The following outline is used for general planning of a crystallography project:
• Protein purification: concentrated protein > 95% pure
• Crystallization: 3D, mountable crystals
• Crystal screening: search for high quality, high resolution crystals
• Data collection: using facility or synchrotron equipment
• Structure determination: processing, phasing, refinement, modeling, analysis, deposition
Additional information can be found at http://www.biotech.iastate.edu/facilities/XRAY/xray.html
Fees for Services and Terms of Use
On Campus Manager time $16.75/hour
Synchrotron trips $270.00
X-ray machine $6.50/hour
Liquid nitrogen $5.00/hour
Archiving $15.00
Shelving $5.00
Off Campus
Manager time $35.70/hour
Synchrotron trips $575.00
X-ray machine $10.90/hour
Liquid nitrogen $5.35/hour
Archiving $22.00
Shelving $5.30
Supplies available upon request for market price.
Crystallography services are open to Iowa State University clients and off-campus individuals or companies.
Fees are subject to change.
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