Polk Hall Room 01, 02, 04
Phone: 919-513-0173
Fax: 919-515-2047
Principle Investigators:
Dr. Carla Mattos Email
Dr. Robert Rose Email
Dr. Flora Meilleur Email
Manager:
Paul Swartz Email
XRC Orientation
XRC Out of Department Use Policy
SER-CAT-Remote-Server-Tutorial
Biological X-ray Crystallography:
Macromolecular x-ray crystallography is a means of determining the 3-dimensional structure of a macromolecule from the systematic scattering of x-rays by a crystal made of that macromolecule. Bio-macromolecules include protein, DNA, RNA, and combinations of the three. The process of x-ray crystallography requires X-rays that are generated via electron acceleration towards a copper anode which excites copper atoms to a higher energy state and which emit x-rays when returning to their ground state. The crystal is positioned in the x-ray beam and diffracted x-rays are detected using an image plate detector. Data analysis and processing followed by modeling of the data yield the 3-dimensional structure. The 3-dimensional structure of the macromolecule is used to study the details of the relationship between the structure of the biological macromolecule and its function. Additionally, the 3-dimensional models of macromolecules are used in the intelligent design of pharmaceuticals and pesticides as well as to understand the nature of metabolic diseases.
Facilities:
X-ray Generators:
Rigaku RuH2R rotating anode x-ray generator (Copper, 1.54 Ã…) with Osmic Confocal X-ray Optics, Rayonix/MAR MAR345 image plate detector and Oxford Instruments Cryojet.
GEC Avionics (Elliot X-ray) GX-13 rotating anode x-ray generator (Copper, 1.54 A) with Osmic Confocal X-ray Optics, Rayonix/MAR MAR345 image plate detector and Oxford Instruments Cryojet.
Detector Control and Data Acquisition:
Shutter control, cryojet control, and detector control from control room. Dell Core 2 Duo computers with 350GB hard drive and dual monitors. Data reduction with HKL or MOSFLM.
Rigaku RuH2R GEC Avionics GX-13
Sample Preparation:
Sample preparation area in control room (Polk 04).
Growth Chamber:
Two walk-in crystal growth chamber with vibration damping cradles for crystal tray storage, one at 18° C, the other at 4° C.
Computer Lab:
Consists of 9 Linux workstations for solving crystal structures and structural analysis. Model building with COOT and optimization with CNS or CCP4.
Remote Crystal Data Collection – Collecting diffraction data from SER-CAT via robotic control from NCSU. (NCSU is a member of SER-CAT – Southeast Regional Collaborative Access Team, APS, ANL, http://www.ser-cat.org/index.html)
128 Polk Hall Raleigh, NC 27695-7622
Telephone: 919-515-5801 Fax: 919-515-2047
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